本文主要研究内容
作者赵丹(2019)在《白术多糖对环磷酰胺诱导的雏鸡肝脏自噬与凋亡的影响》一文中研究指出:本试验在成功建立雏鸡肝脏损伤模型的基础上,从细胞自噬与凋亡的角度探讨白术多糖(Polysaccharide of Atractylodes Macrocephala Koidz,PAMK)对环磷酰胺(cyclophosphamide,CTX)诱导的雏鸡肝脏损伤的影响,应用酶联免疫吸附(ELISA)法检测雏鸡血清中免疫球蛋白含量、血清及肝脏中细胞因子含量,应用光镜和透射电镜对雏鸡肝脏进行形态学观察,应用实时荧光定量PCR法(Realtime PCR)和免疫印迹法(Western Blot)检测与细胞自噬及凋亡相关基因mRNA表达量和蛋白含量。研究结果如下:(1)血清中免疫球蛋白和细胞因子含量的研究结果显示:与对照组相比,环磷酰胺组雏鸡血清中免疫球蛋白(IgM和IgA)和细胞因子(IL-4和IL-6)含量显著降低(P<0.05)。与环磷酰胺组相比,白术多糖环磷酰胺联合组雏鸡血清中(IgM和IgA)和IL-6含量均显著升高(P<0.05)。该研究结果表明,环磷酰胺可导致雏鸡血清中免疫球蛋白和细胞因子含量下降,而白术多糖可缓解雏鸡血清中免疫球蛋白和细胞因子含量的下降。(2)肝脏中细胞因子含量的研究结果显示:与对照组相比,环磷酰胺组雏鸡肝脏中(IL-2、IL-4和IL-6)含量显著降低(P<0.05)。与环磷酰胺组相比,白术多糖环磷酰胺联合组雏鸡肝脏中(IL-2和IL-4)含量显著升高(P<0.05)。该研究结果表明,环磷酰胺可降低雏鸡肝脏中细胞因子含量,而白术多糖可促进肝脏细胞因子的分泌。(3)光镜结果显示:与对照组相比,环磷酰胺可破坏雏鸡肝脏细胞形态结构的完整性、使肝细胞索排列紊乱、并出现炎性细胞浸润。电镜结果显示:与对照组相比,环磷酰胺可导致雏鸡肝脏线粒体受损,并出现自噬小体和凋亡小体。该研究结果表明,自噬与凋亡参与肝脏损伤的病理过程。(4)对自噬相关通路的研究结果显示:与对照组相比,环磷酰胺组雏鸡肝脏中LC3-Ⅰ、LC3-Ⅱ、LC3-Ⅱ/LC3-Ⅰ、Beclin1、Dynein、ATG5和TOR的mRNA表达量显著增加(P<0.05),PI3K和AKT的mRNA表达量显著降低(P<0.05);同时Beclin1、Dynein和ATG5的蛋白含量也显著增加(P<0.05)。与环磷酰胺组相比,白术多糖环磷酰胺联合组雏鸡肝脏中LC3-Ⅱ、LC3-Ⅱ/LC3-Ⅰ和ATG5的mRNA表达量显著降低(P<0.05),PI3K和AKT的mRNA表达量显著增加(P<0.05)。该研究结果表明,环磷酰胺可诱导雏鸡肝脏发生自噬,而白术多糖能够缓解环磷酰胺诱导的雏鸡肝脏自噬的发生。(5)对凋亡中线粒体通路的研究结果显示:与对照组相比,环磷酰胺组雏鸡肝脏中促凋亡基因(Caspase-3、Caspase-9、Bax和p53)mRNA表达量显著增加(P<0.05),抑凋亡基因Bcl-2 mRNA表达量显著下降(P<0.05);同时Caspase-3、Caspase-9和Bax的蛋白含量显著升高(P<0.05),抑凋亡基因Bcl-2的蛋白含量显著降低(P<0.05)。与环磷酰胺组相比,白术多糖环磷酰胺联合组雏鸡肝脏中Caspase-3、Bax和抑凋亡基因Bcl-2的mRNA表达量均呈现出与环磷酰胺组相反的趋势(P<0.05)。此外,Caspase-3和Bcl-2蛋白表达水平的变化趋势和它们相应的mRNA表达水平变化趋势是一致的。该研究结果表明,线粒体通路参与了环磷酰胺诱导的雏鸡肝脏细胞凋亡,而白术多糖可通过调节线粒体通路关键基因及蛋白的表达缓解环磷酰胺诱导的雏鸡肝脏细胞凋亡。(6)对凋亡中内质网应激通路的研究结果显示:与对照组相比,环磷酰胺组雏鸡肝脏中ATF4、ATF6和GRP94 mRNA表达量显著增加(P<0.05);同时雏鸡肝脏中GRP94的蛋白含量也显著增加(P<0.05)。与环磷酰胺组相比,白术多糖环磷酰胺联合组雏鸡肝脏中ATF4和GRP94mRNA表达量和GRP94蛋白含量均显著降低(P<0.05)。该研究结果表明,内质网应激通路参与了环磷酰胺诱导的雏鸡肝脏细胞凋亡,而白术多糖可通过调节内质网通路关键基因及蛋白的表达缓解环磷酰胺诱导的雏鸡肝脏细胞凋亡。(7)对凋亡中死亡受体通路的研究结果显示:与对照组相比,环磷酰胺组雏鸡肝脏中FADD、Fas和Caspase-8的mRNA表达量显著增加(P<0.05);同时FADD和Caspase-8的蛋白含量也显著升高(P<0.05)。与环磷酰胺组相比,白术多糖环磷酰胺联合组雏鸡肝脏中Fas和Caspase-8的mRNA表达量显著降低(P<0.05);白术多糖环磷酰胺联合组雏鸡肝脏中FADD和Caspase-8蛋白含量显著降低(P<0.05)。该研究结果表明,死亡受体通路参与了环磷酰胺诱导的雏鸡肝脏细胞凋亡,而白术多糖可通过调节死亡受体通路关键基因及蛋白的表达缓解环磷酰胺诱导的雏鸡肝脏细胞凋亡。综上所述,环磷酰胺可导致雏鸡血清和肝脏组织中与免疫相关细胞因子含量下降、肝脏发生自噬和凋亡等形态学损伤,而白术多糖可以通过促进细胞因子的分泌、调节与自噬和凋亡相关基因和蛋白的表达来缓解该损伤。
Abstract
ben shi yan zai cheng gong jian li chu ji gan zang sun shang mo xing de ji chu shang ,cong xi bao zi shi yu diao wang de jiao du tan tao bai shu duo tang (Polysaccharide of Atractylodes Macrocephala Koidz,PAMK)dui huan lin xian an (cyclophosphamide,CTX)you dao de chu ji gan zang sun shang de ying xiang ,ying yong mei lian mian yi xi fu (ELISA)fa jian ce chu ji xie qing zhong mian yi qiu dan bai han liang 、xie qing ji gan zang zhong xi bao yin zi han liang ,ying yong guang jing he tou she dian jing dui chu ji gan zang jin hang xing tai xue guan cha ,ying yong shi shi ying guang ding liang PCRfa (Realtime PCR)he mian yi yin ji fa (Western Blot)jian ce yu xi bao zi shi ji diao wang xiang guan ji yin mRNAbiao da liang he dan bai han liang 。yan jiu jie guo ru xia :(1)xie qing zhong mian yi qiu dan bai he xi bao yin zi han liang de yan jiu jie guo xian shi :yu dui zhao zu xiang bi ,huan lin xian an zu chu ji xie qing zhong mian yi qiu dan bai (IgMhe IgA)he xi bao yin zi (IL-4he IL-6)han liang xian zhe jiang di (P<0.05)。yu huan lin xian an zu xiang bi ,bai shu duo tang huan lin xian an lian ge zu chu ji xie qing zhong (IgMhe IgA)he IL-6han liang jun xian zhe sheng gao (P<0.05)。gai yan jiu jie guo biao ming ,huan lin xian an ke dao zhi chu ji xie qing zhong mian yi qiu dan bai he xi bao yin zi han liang xia jiang ,er bai shu duo tang ke huan jie chu ji xie qing zhong mian yi qiu dan bai he xi bao yin zi han liang de xia jiang 。(2)gan zang zhong xi bao yin zi han liang de yan jiu jie guo xian shi :yu dui zhao zu xiang bi ,huan lin xian an zu chu ji gan zang zhong (IL-2、IL-4he IL-6)han liang xian zhe jiang di (P<0.05)。yu huan lin xian an zu xiang bi ,bai shu duo tang huan lin xian an lian ge zu chu ji gan zang zhong (IL-2he IL-4)han liang xian zhe sheng gao (P<0.05)。gai yan jiu jie guo biao ming ,huan lin xian an ke jiang di chu ji gan zang zhong xi bao yin zi han liang ,er bai shu duo tang ke cu jin gan zang xi bao yin zi de fen bi 。(3)guang jing jie guo xian shi :yu dui zhao zu xiang bi ,huan lin xian an ke po huai chu ji gan zang xi bao xing tai jie gou de wan zheng xing 、shi gan xi bao suo pai lie wen luan 、bing chu xian yan xing xi bao jin run 。dian jing jie guo xian shi :yu dui zhao zu xiang bi ,huan lin xian an ke dao zhi chu ji gan zang xian li ti shou sun ,bing chu xian zi shi xiao ti he diao wang xiao ti 。gai yan jiu jie guo biao ming ,zi shi yu diao wang can yu gan zang sun shang de bing li guo cheng 。(4)dui zi shi xiang guan tong lu de yan jiu jie guo xian shi :yu dui zhao zu xiang bi ,huan lin xian an zu chu ji gan zang zhong LC3-Ⅰ、LC3-Ⅱ、LC3-Ⅱ/LC3-Ⅰ、Beclin1、Dynein、ATG5he TORde mRNAbiao da liang xian zhe zeng jia (P<0.05),PI3Khe AKTde mRNAbiao da liang xian zhe jiang di (P<0.05);tong shi Beclin1、Dyneinhe ATG5de dan bai han liang ye xian zhe zeng jia (P<0.05)。yu huan lin xian an zu xiang bi ,bai shu duo tang huan lin xian an lian ge zu chu ji gan zang zhong LC3-Ⅱ、LC3-Ⅱ/LC3-Ⅰhe ATG5de mRNAbiao da liang xian zhe jiang di (P<0.05),PI3Khe AKTde mRNAbiao da liang xian zhe zeng jia (P<0.05)。gai yan jiu jie guo biao ming ,huan lin xian an ke you dao chu ji gan zang fa sheng zi shi ,er bai shu duo tang neng gou huan jie huan lin xian an you dao de chu ji gan zang zi shi de fa sheng 。(5)dui diao wang zhong xian li ti tong lu de yan jiu jie guo xian shi :yu dui zhao zu xiang bi ,huan lin xian an zu chu ji gan zang zhong cu diao wang ji yin (Caspase-3、Caspase-9、Baxhe p53)mRNAbiao da liang xian zhe zeng jia (P<0.05),yi diao wang ji yin Bcl-2 mRNAbiao da liang xian zhe xia jiang (P<0.05);tong shi Caspase-3、Caspase-9he Baxde dan bai han liang xian zhe sheng gao (P<0.05),yi diao wang ji yin Bcl-2de dan bai han liang xian zhe jiang di (P<0.05)。yu huan lin xian an zu xiang bi ,bai shu duo tang huan lin xian an lian ge zu chu ji gan zang zhong Caspase-3、Baxhe yi diao wang ji yin Bcl-2de mRNAbiao da liang jun cheng xian chu yu huan lin xian an zu xiang fan de qu shi (P<0.05)。ci wai ,Caspase-3he Bcl-2dan bai biao da shui ping de bian hua qu shi he ta men xiang ying de mRNAbiao da shui ping bian hua qu shi shi yi zhi de 。gai yan jiu jie guo biao ming ,xian li ti tong lu can yu le huan lin xian an you dao de chu ji gan zang xi bao diao wang ,er bai shu duo tang ke tong guo diao jie xian li ti tong lu guan jian ji yin ji dan bai de biao da huan jie huan lin xian an you dao de chu ji gan zang xi bao diao wang 。(6)dui diao wang zhong nei zhi wang ying ji tong lu de yan jiu jie guo xian shi :yu dui zhao zu xiang bi ,huan lin xian an zu chu ji gan zang zhong ATF4、ATF6he GRP94 mRNAbiao da liang xian zhe zeng jia (P<0.05);tong shi chu ji gan zang zhong GRP94de dan bai han liang ye xian zhe zeng jia (P<0.05)。yu huan lin xian an zu xiang bi ,bai shu duo tang huan lin xian an lian ge zu chu ji gan zang zhong ATF4he GRP94mRNAbiao da liang he GRP94dan bai han liang jun xian zhe jiang di (P<0.05)。gai yan jiu jie guo biao ming ,nei zhi wang ying ji tong lu can yu le huan lin xian an you dao de chu ji gan zang xi bao diao wang ,er bai shu duo tang ke tong guo diao jie nei zhi wang tong lu guan jian ji yin ji dan bai de biao da huan jie huan lin xian an you dao de chu ji gan zang xi bao diao wang 。(7)dui diao wang zhong si wang shou ti tong lu de yan jiu jie guo xian shi :yu dui zhao zu xiang bi ,huan lin xian an zu chu ji gan zang zhong FADD、Fashe Caspase-8de mRNAbiao da liang xian zhe zeng jia (P<0.05);tong shi FADDhe Caspase-8de dan bai han liang ye xian zhe sheng gao (P<0.05)。yu huan lin xian an zu xiang bi ,bai shu duo tang huan lin xian an lian ge zu chu ji gan zang zhong Fashe Caspase-8de mRNAbiao da liang xian zhe jiang di (P<0.05);bai shu duo tang huan lin xian an lian ge zu chu ji gan zang zhong FADDhe Caspase-8dan bai han liang xian zhe jiang di (P<0.05)。gai yan jiu jie guo biao ming ,si wang shou ti tong lu can yu le huan lin xian an you dao de chu ji gan zang xi bao diao wang ,er bai shu duo tang ke tong guo diao jie si wang shou ti tong lu guan jian ji yin ji dan bai de biao da huan jie huan lin xian an you dao de chu ji gan zang xi bao diao wang 。zeng shang suo shu ,huan lin xian an ke dao zhi chu ji xie qing he gan zang zu zhi zhong yu mian yi xiang guan xi bao yin zi han liang xia jiang 、gan zang fa sheng zi shi he diao wang deng xing tai xue sun shang ,er bai shu duo tang ke yi tong guo cu jin xi bao yin zi de fen bi 、diao jie yu zi shi he diao wang xiang guan ji yin he dan bai de biao da lai huan jie gai sun shang 。
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论文作者分别是来自东北农业大学的赵丹,发表于刊物东北农业大学2019-08-27论文,是一篇关于白术多糖论文,环磷酰胺论文,雏鸡肝脏损伤论文,自噬论文,凋亡论文,东北农业大学2019-08-27论文的文章。本文可供学术参考使用,各位学者可以免费参考阅读下载,文章观点不代表本站观点,资料来自东北农业大学2019-08-27论文网站,若本站收录的文献无意侵犯了您的著作版权,请联系我们删除。
标签:白术多糖论文; 环磷酰胺论文; 雏鸡肝脏损伤论文; 自噬论文; 凋亡论文; 东北农业大学2019-08-27论文;