本文主要研究内容
作者史宏昭（2019）在《冷暴露小鼠腓肠肌RBM3上调影响OGT介导的糖酵解》一文中研究指出：环境低温显著诱导机体基础代谢和免疫功能改变。由于家猪UCP1基因丢失导致缺乏功能性BAT产热应对环境低温。数据显示,仔猪病毒或者细菌性腹泻可能由保育舍低温导致。而且仔猪养殖供暖能源花费巨大。因此,研究低温下仔猪重要产热器官肌肉冷响应机制,对于提高仔猪低温抵抗力,降低猪养殖业能源成本有重要意义。课题组前期研究发现,低温下仔猪血糖稳定期间,腓肠肌糖酵解代谢发生显著变化与腓肠肌冷响应蛋白RBM3和应激感受器蛋白OGT表达变化同步。本研究以小鼠为动物模型,研究相关信号通路和生化途径响应变化。为检测冷暴露对小鼠血糖和胰岛素影响,本研究通过检测冷暴露禁食2 h和4 h小鼠血清显示与常温对照组相比,冷暴露期间血糖保持稳定,而胰岛素在冷暴露期间成波动状。冷暴露影响小鼠腓肠肌糖原和ATP含量,结果显示冷暴露4 h小鼠腓肠肌肌糖原和ATP含量与常温对照组显著下降。同时检测PI3K/AKT糖酵解、葡萄糖转运和糖原合成通路相关蛋白表达。结果显示,冷暴露4 h小鼠腓肠肌AKT/GSK3β/GS,AKT/P-PFKFB2,p65磷酸化显著升高,以及OGT、RBM3、Glut4、Bcl-2/Bax蛋白表达水平显著上调。而冷暴露4 h小鼠腓肠肌Cleaved-Caspase3表达显著下调。这些结果潜在表明小鼠腓肠肌通过调节冷暴露4 h时OGT、RBM3、Glut4、Bcl-2/Bax等蛋白表达和PI3K/AKT糖酵解、葡萄糖转运以及糖原合成响应冷暴露。本研究使用AKT抑制剂渥曼青霉素验证是否影响冷暴露4 h下腓肠肌冷响应相关蛋白表达变化。结果显示,小鼠腓肠IRS-1磷酸化在冷暴露4 h下对照组、AKT抑制剂组和DMSO组之间无显著变化,而Cleaved-Caspase 3显著升高与Bcl-2/Bax表达相反;RBM3蛋白表达水平和AKT/GSK3β/GS,AKT/P-PFKFB2和AKT/p65磷酸化显著下降。为进一步研究RBM3、p65、AKT和OGT之间是否存在串扰,研究采用慢病毒载体在C2C12小鼠成肌细胞过表达RBM3蛋白。结果显示,相比野生型和空质粒对照组C2C12细胞,过表达RBM3组在常温下和4℃冷暴露下显著促进AKT磷酸化。同时,条件性敲除小鼠骨骼肌OGT。结果显示,OGT~-/~-敲除小鼠与同窝野生型小鼠相比AKT、GSK 3β、PFKFB2磷酸化和Cleaved-Caspase 3表达显著升高。而p65、GS磷酸化、RBM3与Bcl-2/Bax表达显著下降。同时检测到冷暴露4 h糖酵解中产物FDP和PA在OGT敲除小鼠腓肠肌中累积。这些结果表明存在OGT介导RBM3表达促进AKT磷酸化以及OGT介导AKT糖酵解通量。依据以上结果,本研究发现:冷暴露小鼠腓肠肌OGT介导RBM3上调;冷暴露小鼠腓肠肌上调RBM3促进AKT磷酸化参与细胞保护作用以及糖酵解;冷暴露小鼠腓肠肌OGT介导胰岛素糖酵解生化途径。
Abstract
huan jing di wen xian zhe you dao ji ti ji chu dai xie he mian yi gong neng gai bian 。you yu jia zhu UCP1ji yin diu shi dao zhi que fa gong neng xing BATchan re ying dui huan jing di wen 。shu ju xian shi ,zai zhu bing du huo zhe xi jun xing fu xie ke neng you bao yo she di wen dao zhi 。er ju zai zhu yang shi gong nuan neng yuan hua fei ju da 。yin ci ,yan jiu di wen xia zai zhu chong yao chan re qi guan ji rou leng xiang ying ji zhi ,dui yu di gao zai zhu di wen di kang li ,jiang di zhu yang shi ye neng yuan cheng ben you chong yao yi yi 。ke ti zu qian ji yan jiu fa xian ,di wen xia zai zhu xie tang wen ding ji jian ,fei chang ji tang jiao jie dai xie fa sheng xian zhe bian hua yu fei chang ji leng xiang ying dan bai RBM3he ying ji gan shou qi dan bai OGTbiao da bian hua tong bu 。ben yan jiu yi xiao shu wei dong wu mo xing ,yan jiu xiang guan xin hao tong lu he sheng hua tu jing xiang ying bian hua 。wei jian ce leng bao lou dui xiao shu xie tang he yi dao su ying xiang ,ben yan jiu tong guo jian ce leng bao lou jin shi 2 hhe 4 hxiao shu xie qing xian shi yu chang wen dui zhao zu xiang bi ,leng bao lou ji jian xie tang bao chi wen ding ,er yi dao su zai leng bao lou ji jian cheng bo dong zhuang 。leng bao lou ying xiang xiao shu fei chang ji tang yuan he ATPhan liang ,jie guo xian shi leng bao lou 4 hxiao shu fei chang ji ji tang yuan he ATPhan liang yu chang wen dui zhao zu xian zhe xia jiang 。tong shi jian ce PI3K/AKTtang jiao jie 、pu tao tang zhuai yun he tang yuan ge cheng tong lu xiang guan dan bai biao da 。jie guo xian shi ,leng bao lou 4 hxiao shu fei chang ji AKT/GSK3β/GS,AKT/P-PFKFB2,p65lin suan hua xian zhe sheng gao ,yi ji OGT、RBM3、Glut4、Bcl-2/Baxdan bai biao da shui ping xian zhe shang diao 。er leng bao lou 4 hxiao shu fei chang ji Cleaved-Caspase3biao da xian zhe xia diao 。zhe xie jie guo qian zai biao ming xiao shu fei chang ji tong guo diao jie leng bao lou 4 hshi OGT、RBM3、Glut4、Bcl-2/Baxdeng dan bai biao da he PI3K/AKTtang jiao jie 、pu tao tang zhuai yun yi ji tang yuan ge cheng xiang ying leng bao lou 。ben yan jiu shi yong AKTyi zhi ji wo man qing mei su yan zheng shi fou ying xiang leng bao lou 4 hxia fei chang ji leng xiang ying xiang guan dan bai biao da bian hua 。jie guo xian shi ,xiao shu fei chang IRS-1lin suan hua zai leng bao lou 4 hxia dui zhao zu 、AKTyi zhi ji zu he DMSOzu zhi jian mo xian zhe bian hua ,er Cleaved-Caspase 3xian zhe sheng gao yu Bcl-2/Baxbiao da xiang fan ;RBM3dan bai biao da shui ping he AKT/GSK3β/GS,AKT/P-PFKFB2he AKT/p65lin suan hua xian zhe xia jiang 。wei jin yi bu yan jiu RBM3、p65、AKThe OGTzhi jian shi fou cun zai chuan rao ,yan jiu cai yong man bing du zai ti zai C2C12xiao shu cheng ji xi bao guo biao da RBM3dan bai 。jie guo xian shi ,xiang bi ye sheng xing he kong zhi li dui zhao zu C2C12xi bao ,guo biao da RBM3zu zai chang wen xia he 4℃leng bao lou xia xian zhe cu jin AKTlin suan hua 。tong shi ,tiao jian xing qiao chu xiao shu gu ge ji OGT。jie guo xian shi ,OGT~-/~-qiao chu xiao shu yu tong wo ye sheng xing xiao shu xiang bi AKT、GSK 3β、PFKFB2lin suan hua he Cleaved-Caspase 3biao da xian zhe sheng gao 。er p65、GSlin suan hua 、RBM3yu Bcl-2/Baxbiao da xian zhe xia jiang 。tong shi jian ce dao leng bao lou 4 htang jiao jie zhong chan wu FDPhe PAzai OGTqiao chu xiao shu fei chang ji zhong lei ji 。zhe xie jie guo biao ming cun zai OGTjie dao RBM3biao da cu jin AKTlin suan hua yi ji OGTjie dao AKTtang jiao jie tong liang 。yi ju yi shang jie guo ,ben yan jiu fa xian :leng bao lou xiao shu fei chang ji OGTjie dao RBM3shang diao ;leng bao lou xiao shu fei chang ji shang diao RBM3cu jin AKTlin suan hua can yu xi bao bao hu zuo yong yi ji tang jiao jie ;leng bao lou xiao shu fei chang ji OGTjie dao yi dao su tang jiao jie sheng hua tu jing 。
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论文详细介绍
论文作者分别是来自黑龙江八一农垦大学的史宏昭，发表于刊物黑龙江八一农垦大学2019-07-08论文，是一篇关于冷暴露论文,腓肠肌论文,糖酵解论文,黑龙江八一农垦大学2019-07-08论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自黑龙江八一农垦大学2019-07-08论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：冷暴露论文; 腓肠肌论文; 糖酵解论文; 黑龙江八一农垦大学2019-07-08论文;