潘双子:食用菌多糖对人工瘤胃发酵及甲烷产量的影响论文

潘双子:食用菌多糖对人工瘤胃发酵及甲烷产量的影响论文

本文主要研究内容

作者潘双子(2019)在《食用菌多糖对人工瘤胃发酵及甲烷产量的影响》一文中研究指出:瘤胃中CH4的产生不仅导致温室效应问题加剧,也降低了饲料的利用率,所以降低反刍动物瘤胃CH4产量受到广大学者的关注。本论文研究了食用菌多糖对山羊瘤胃发酵、CH4、酶活性及瘤胃微生物的影响,探讨了其作用机理,旨在为开发食用菌多糖作为瘤胃产甲烷菌抑制剂提供理论依据。实验一:食用菌多糖对人工瘤胃发酵的影响。采用体外人工瘤胃产气法进行体外发酵实验,分析食用菌多糖对人工瘤胃发酵参数的影响。结果表明:125500 mg/kg的香菇多糖、5 mg/kg的灵芝多糖可促进人工瘤胃发酵,能显著提高GP产量(P<0.05),虫草多糖、猴头菇多糖及猪苓多糖对人工瘤胃GP产量无显著影响(P>0.05);5500 mg/kg香菇多糖、525 mg/kg灵芝多糖和125 mg/kg虫草多糖可显著提高人工瘤胃发酵液pH值(P<0.05),猴头菇多糖和猪苓多糖对人工瘤胃pH值无显著影响(P>0.05);25125 mg/kg香菇多糖、5 mg/kg灵芝多糖、125500 mg/kg虫草多糖、125500 mg/kg猴头菇多糖可显著提高人工瘤胃NH3-N浓度,猪苓多糖对人工瘤胃NH3-N浓度的影响差异不显著(P>0.05);香菇多糖、灵芝多糖和虫草多糖可以显著提高人工瘤胃TVFA、乙酸和丙酸浓度(P<0.05),其中香菇多糖和虫草多糖可降低乙酸/丙酸比值,瘤胃发酵模式由乙酸型发酵模式趋向于丙酸型发酵模式。添加虫草多糖的丙酸浓度显著高于其他添加组(P<0.05)。综合分析,添加125500 mg/kg的香菇多糖、5 mg/kg的灵芝多糖和125 mg/kg虫草多糖可显著改善瘤胃发酵模式。实验二:食用菌多糖对人工瘤胃CH4产量及酶活性的影响。结果表明:500 mg/kg香菇多糖、5 mg/kg灵芝多糖、125 mg/kg虫草多糖组人工瘤胃辅酶F420活性较对照组分别降低70.2%(P<0.05)、40.3%(P<0.05)、53.6%(P<0.05);125 mg/kg香菇多糖、5 mg/kg灵芝多糖、25 mg/kg虫草多糖组人工瘤胃辅酶M(CoM-S-H)活性较对照组分别降低37.1%(P<0.05)、52.4%(P<0.05)、33.9%(P<0.05);香菇多糖、灵芝多糖及虫草多糖组人工瘤胃CH4产量较对照组显著降低(P<0.05),其中,25 mg/kg香菇多糖组降低10.4%(P<0.05),5 mg/kg灵芝多糖组降低13.6%(P<0.05),125 mg/kg虫草多糖组降低6.9%(P<0.05)。添加水平为S1时,灵芝多糖组的CH4浓度显著低于其他多糖组(P<0.05);添加水平为S3时,香菇多糖、灵芝多糖和虫草多糖组的CO2浓度显著低于猴头菇多糖和猪苓多糖组(P<0.05)。综合分析,添加香菇、灵芝和虫草多糖均可显著降低人工瘤胃各个辅酶的活性,且降低CH4产量。实验三:食用菌多糖对人工发酵瘤胃微生物菌群的影响。采用实时荧光定量PCR法,测定不同添加水平的香菇、灵芝和虫草多糖对山羊瘤胃微生物的影响。结果表明:香菇多糖、灵芝多糖及虫草多糖可显著增加人工瘤胃总细菌相对数量(P<0.05);125 mg/kg香菇多糖、5 mg/kg灵芝多糖及525 mg/kg虫草多糖实验组的人工瘤胃原虫相对数量显著低于对照组(P<0.05);125500 mg/kg香菇多糖及125 mg/kg虫草多糖实验组的人工瘤胃真菌相对数量显著高于对照组(P<0.05);25125 mg/kg香菇多糖、5 mg/kg灵芝多糖及125 mg/kg虫草多糖实验组的人工瘤胃甲烷菌相对数量显著低于对照组(P<0.05)。添加水平为S1时,灵芝多糖组的产甲烷菌相对数量显著低于其他多糖组(P<0.05);添加水平为S3时,香菇多糖组产甲烷菌相对数量显著低于其他多糖组(P<0.05)。综合分析,添加香菇、灵芝和虫草多糖均可提高瘤胃总细菌的相对数量,且降低原虫和产甲烷菌的相对数量。

Abstract

liu wei zhong CH4de chan sheng bu jin dao zhi wen shi xiao ying wen ti jia ju ,ye jiang di le si liao de li yong lv ,suo yi jiang di fan chu dong wu liu wei CH4chan liang shou dao an da xue zhe de guan zhu 。ben lun wen yan jiu le shi yong jun duo tang dui shan yang liu wei fa jiao 、CH4、mei huo xing ji liu wei wei sheng wu de ying xiang ,tan tao le ji zuo yong ji li ,zhi zai wei kai fa shi yong jun duo tang zuo wei liu wei chan jia wan jun yi zhi ji di gong li lun yi ju 。shi yan yi :shi yong jun duo tang dui ren gong liu wei fa jiao de ying xiang 。cai yong ti wai ren gong liu wei chan qi fa jin hang ti wai fa jiao shi yan ,fen xi shi yong jun duo tang dui ren gong liu wei fa jiao can shu de ying xiang 。jie guo biao ming :125500 mg/kgde xiang gu duo tang 、5 mg/kgde ling zhi duo tang ke cu jin ren gong liu wei fa jiao ,neng xian zhe di gao GPchan liang (P<0.05),chong cao duo tang 、hou tou gu duo tang ji zhu ling duo tang dui ren gong liu wei GPchan liang mo xian zhe ying xiang (P>0.05);5500 mg/kgxiang gu duo tang 、525 mg/kgling zhi duo tang he 125 mg/kgchong cao duo tang ke xian zhe di gao ren gong liu wei fa jiao ye pHzhi (P<0.05),hou tou gu duo tang he zhu ling duo tang dui ren gong liu wei pHzhi mo xian zhe ying xiang (P>0.05);25125 mg/kgxiang gu duo tang 、5 mg/kgling zhi duo tang 、125500 mg/kgchong cao duo tang 、125500 mg/kghou tou gu duo tang ke xian zhe di gao ren gong liu wei NH3-Nnong du ,zhu ling duo tang dui ren gong liu wei NH3-Nnong du de ying xiang cha yi bu xian zhe (P>0.05);xiang gu duo tang 、ling zhi duo tang he chong cao duo tang ke yi xian zhe di gao ren gong liu wei TVFA、yi suan he bing suan nong du (P<0.05),ji zhong xiang gu duo tang he chong cao duo tang ke jiang di yi suan /bing suan bi zhi ,liu wei fa jiao mo shi you yi suan xing fa jiao mo shi qu xiang yu bing suan xing fa jiao mo shi 。tian jia chong cao duo tang de bing suan nong du xian zhe gao yu ji ta tian jia zu (P<0.05)。zeng ge fen xi ,tian jia 125500 mg/kgde xiang gu duo tang 、5 mg/kgde ling zhi duo tang he 125 mg/kgchong cao duo tang ke xian zhe gai shan liu wei fa jiao mo shi 。shi yan er :shi yong jun duo tang dui ren gong liu wei CH4chan liang ji mei huo xing de ying xiang 。jie guo biao ming :500 mg/kgxiang gu duo tang 、5 mg/kgling zhi duo tang 、125 mg/kgchong cao duo tang zu ren gong liu wei fu mei F420huo xing jiao dui zhao zu fen bie jiang di 70.2%(P<0.05)、40.3%(P<0.05)、53.6%(P<0.05);125 mg/kgxiang gu duo tang 、5 mg/kgling zhi duo tang 、25 mg/kgchong cao duo tang zu ren gong liu wei fu mei M(CoM-S-H)huo xing jiao dui zhao zu fen bie jiang di 37.1%(P<0.05)、52.4%(P<0.05)、33.9%(P<0.05);xiang gu duo tang 、ling zhi duo tang ji chong cao duo tang zu ren gong liu wei CH4chan liang jiao dui zhao zu xian zhe jiang di (P<0.05),ji zhong ,25 mg/kgxiang gu duo tang zu jiang di 10.4%(P<0.05),5 mg/kgling zhi duo tang zu jiang di 13.6%(P<0.05),125 mg/kgchong cao duo tang zu jiang di 6.9%(P<0.05)。tian jia shui ping wei S1shi ,ling zhi duo tang zu de CH4nong du xian zhe di yu ji ta duo tang zu (P<0.05);tian jia shui ping wei S3shi ,xiang gu duo tang 、ling zhi duo tang he chong cao duo tang zu de CO2nong du xian zhe di yu hou tou gu duo tang he zhu ling duo tang zu (P<0.05)。zeng ge fen xi ,tian jia xiang gu 、ling zhi he chong cao duo tang jun ke xian zhe jiang di ren gong liu wei ge ge fu mei de huo xing ,ju jiang di CH4chan liang 。shi yan san :shi yong jun duo tang dui ren gong fa jiao liu wei wei sheng wu jun qun de ying xiang 。cai yong shi shi ying guang ding liang PCRfa ,ce ding bu tong tian jia shui ping de xiang gu 、ling zhi he chong cao duo tang dui shan yang liu wei wei sheng wu de ying xiang 。jie guo biao ming :xiang gu duo tang 、ling zhi duo tang ji chong cao duo tang ke xian zhe zeng jia ren gong liu wei zong xi jun xiang dui shu liang (P<0.05);125 mg/kgxiang gu duo tang 、5 mg/kgling zhi duo tang ji 525 mg/kgchong cao duo tang shi yan zu de ren gong liu wei yuan chong xiang dui shu liang xian zhe di yu dui zhao zu (P<0.05);125500 mg/kgxiang gu duo tang ji 125 mg/kgchong cao duo tang shi yan zu de ren gong liu wei zhen jun xiang dui shu liang xian zhe gao yu dui zhao zu (P<0.05);25125 mg/kgxiang gu duo tang 、5 mg/kgling zhi duo tang ji 125 mg/kgchong cao duo tang shi yan zu de ren gong liu wei jia wan jun xiang dui shu liang xian zhe di yu dui zhao zu (P<0.05)。tian jia shui ping wei S1shi ,ling zhi duo tang zu de chan jia wan jun xiang dui shu liang xian zhe di yu ji ta duo tang zu (P<0.05);tian jia shui ping wei S3shi ,xiang gu duo tang zu chan jia wan jun xiang dui shu liang xian zhe di yu ji ta duo tang zu (P<0.05)。zeng ge fen xi ,tian jia xiang gu 、ling zhi he chong cao duo tang jun ke di gao liu wei zong xi jun de xiang dui shu liang ,ju jiang di yuan chong he chan jia wan jun de xiang dui shu liang 。

论文参考文献

论文详细介绍

论文作者分别是来自福建农林大学的潘双子,发表于刊物福建农林大学2019-08-30论文,是一篇关于食用菌多糖论文,瘤胃发酵论文,辅酶论文,产甲烷菌论文,福建农林大学2019-08-30论文的文章。本文可供学术参考使用,各位学者可以免费参考阅读下载,文章观点不代表本站观点,资料来自福建农林大学2019-08-30论文网站,若本站收录的文献无意侵犯了您的著作版权,请联系我们删除。

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潘双子:食用菌多糖对人工瘤胃发酵及甲烷产量的影响论文
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