本文主要研究内容
作者李飞（2019）在《阴道毛滴虫诱导THP-1细胞产生胞外陷阱的研究》一文中研究指出：阴道毛滴虫在世界范围内分布广泛感染率较高,可能是最常见的通过性传播的人兽共患原虫。阴道毛滴虫一般寄生在人体内,也可以感染猴等动物。人感染阴道毛滴虫后,会产生阴道炎及尿道炎等症状,并且阴道毛滴虫感染与艾滋病病毒、乳头瘤病毒、单纯疱疹病毒的感染有关[1]。胞外陷阱对机体抵御病原入侵具有重要作用,已有研究发现牛艾美尔球虫、新孢子虫、弓形虫等原虫刺激免疫细胞产生了胞外陷阱。胞外陷阱可以捕获入侵的原虫,并且有些胞外陷阱对原虫具有杀伤作用[2]。贾第虫也可刺激免疫细胞产生胞外陷阱[3],但是同为鞭毛虫的阴道毛滴虫是否能刺激细胞产生胞外陷阱并释放活性氧（ROS）等物质阻碍病原入侵,目前尚未见报道。THP-1细胞是人外周血单核细胞系,本试验应用扫描电子显微镜观察了阴道毛滴虫刺激THP-1细胞产生的胞外陷阱;应用激光共聚焦检测了阴道毛滴虫刺激THP-1细胞产生的胞外陷阱的成分;应用活性氧检测试剂盒检测了阴道毛滴虫刺激THP-1细胞产生胞外陷阱时ROS的水平;应用乳酸脱氢酶（LDH）检测试剂盒检测了阴道毛滴虫刺激THP-1细胞产生胞外陷阱时LDH的水平;应用Picogreen双链DNA检测试剂盒检测了刺激时间、刺激比例对阴道毛滴虫刺激THP-1细胞产生胞外陷阱的影响;应用Picogreen双链DNA检测试剂盒检测了不同状态的阴道毛滴虫对阴道毛滴虫刺激THP-1细胞产生胞外陷阱的影响;应用Western blot检测了阴道毛滴虫刺激THP-1细胞产生胞外陷阱时P38MAPK、ERK1/2MAPK信号通路的磷酸化水平;应用IL-1β、TNFα细胞因子检测试剂盒检测了阴道毛滴虫刺激THP-1细胞产生IL-1β、TNFα的水平。扫描电子显微镜结果表明阴道毛滴虫刺激THP-1细胞产生了胞外陷阱,阴道毛滴虫刺激THP-1细胞产生了细长的、具有多个网孔的纤维样网状结构缠绕并捕获了阴道毛滴虫,这直接有效的证明了阴道毛滴虫刺激THP-1细胞产生了胞外陷阱;激光共聚焦结果表明阴道毛滴虫刺激THP-1细胞产生的胞外陷阱具有胞外陷阱的典型结构特征:DNA骨架上镶嵌着组蛋白（H3）、髓过氧化物酶（MPO）;ROS检测结果表明胞外陷阱产生时产生了活性氧,这证明胞外陷阱具有杀伤病原的功能;LDH检测结果表明胞外陷阱产生时THP-1细胞并未发生裂解,这证明胞外陷阱是一种不同于细胞坏死的新的细胞死亡方式;DNA检测结果表明阴道毛滴虫刺激THP-1细胞产生的胞外陷阱具有剂量依赖性,随刺激时间延长逐渐减少,胞外陷阱的产生与阴道毛滴虫的活力没有关系;Western blot检测结果表明阴道毛滴虫刺激THP-1细胞产生胞外陷阱时P38MAPK、ERK1/2MAPK信号通路磷酸化水平上升,这证明了阴道毛滴虫刺激THP-1细胞产生的胞外陷阱与P38MAPK、ERK1/2MAPK信号通路有关;IL-1β、TNFα检测结果表明阴道毛滴虫刺激THP-1细胞产生了IL-1β、TNFα,这证明阴道毛滴虫刺激THP-1细胞产生的胞外陷阱可能与IL-1β、TNFα有关,本研究为探究阴道毛滴虫刺激THP-1细胞产生胞外陷阱的机理提供了新思路。
Abstract
yin dao mao di chong zai shi jie fan wei nei fen bu an fan gan ran lv jiao gao ,ke neng shi zui chang jian de tong guo xing chuan bo de ren shou gong huan yuan chong 。yin dao mao di chong yi ban ji sheng zai ren ti nei ,ye ke yi gan ran hou deng dong wu 。ren gan ran yin dao mao di chong hou ,hui chan sheng yin dao yan ji niao dao yan deng zheng zhuang ,bing ju yin dao mao di chong gan ran yu ai zi bing bing du 、ru tou liu bing du 、chan chun pao zhen bing du de gan ran you guan [1]。bao wai xian jing dui ji ti di yu bing yuan ru qin ju you chong yao zuo yong ,yi you yan jiu fa xian niu ai mei er qiu chong 、xin bao zi chong 、gong xing chong deng yuan chong ci ji mian yi xi bao chan sheng le bao wai xian jing 。bao wai xian jing ke yi bu huo ru qin de yuan chong ,bing ju you xie bao wai xian jing dui yuan chong ju you sha shang zuo yong [2]。gu di chong ye ke ci ji mian yi xi bao chan sheng bao wai xian jing [3],dan shi tong wei bian mao chong de yin dao mao di chong shi fou neng ci ji xi bao chan sheng bao wai xian jing bing shi fang huo xing yang （ROS）deng wu zhi zu ai bing yuan ru qin ,mu qian shang wei jian bao dao 。THP-1xi bao shi ren wai zhou xie chan he xi bao ji ,ben shi yan ying yong sao miao dian zi xian wei jing guan cha le yin dao mao di chong ci ji THP-1xi bao chan sheng de bao wai xian jing ;ying yong ji guang gong ju jiao jian ce le yin dao mao di chong ci ji THP-1xi bao chan sheng de bao wai xian jing de cheng fen ;ying yong huo xing yang jian ce shi ji he jian ce le yin dao mao di chong ci ji THP-1xi bao chan sheng bao wai xian jing shi ROSde shui ping ;ying yong ru suan tuo qing mei （LDH）jian ce shi ji he jian ce le yin dao mao di chong ci ji THP-1xi bao chan sheng bao wai xian jing shi LDHde shui ping ;ying yong Picogreenshuang lian DNAjian ce shi ji he jian ce le ci ji shi jian 、ci ji bi li dui yin dao mao di chong ci ji THP-1xi bao chan sheng bao wai xian jing de ying xiang ;ying yong Picogreenshuang lian DNAjian ce shi ji he jian ce le bu tong zhuang tai de yin dao mao di chong dui yin dao mao di chong ci ji THP-1xi bao chan sheng bao wai xian jing de ying xiang ;ying yong Western blotjian ce le yin dao mao di chong ci ji THP-1xi bao chan sheng bao wai xian jing shi P38MAPK、ERK1/2MAPKxin hao tong lu de lin suan hua shui ping ;ying yong IL-1β、TNFαxi bao yin zi jian ce shi ji he jian ce le yin dao mao di chong ci ji THP-1xi bao chan sheng IL-1β、TNFαde shui ping 。sao miao dian zi xian wei jing jie guo biao ming yin dao mao di chong ci ji THP-1xi bao chan sheng le bao wai xian jing ,yin dao mao di chong ci ji THP-1xi bao chan sheng le xi chang de 、ju you duo ge wang kong de qian wei yang wang zhuang jie gou chan rao bing bu huo le yin dao mao di chong ,zhe zhi jie you xiao de zheng ming le yin dao mao di chong ci ji THP-1xi bao chan sheng le bao wai xian jing ;ji guang gong ju jiao jie guo biao ming yin dao mao di chong ci ji THP-1xi bao chan sheng de bao wai xian jing ju you bao wai xian jing de dian xing jie gou te zheng :DNAgu jia shang rang qian zhao zu dan bai （H3）、sui guo yang hua wu mei （MPO）;ROSjian ce jie guo biao ming bao wai xian jing chan sheng shi chan sheng le huo xing yang ,zhe zheng ming bao wai xian jing ju you sha shang bing yuan de gong neng ;LDHjian ce jie guo biao ming bao wai xian jing chan sheng shi THP-1xi bao bing wei fa sheng lie jie ,zhe zheng ming bao wai xian jing shi yi chong bu tong yu xi bao huai si de xin de xi bao si wang fang shi ;DNAjian ce jie guo biao ming yin dao mao di chong ci ji THP-1xi bao chan sheng de bao wai xian jing ju you ji liang yi lai xing ,sui ci ji shi jian yan chang zhu jian jian shao ,bao wai xian jing de chan sheng yu yin dao mao di chong de huo li mei you guan ji ;Western blotjian ce jie guo biao ming yin dao mao di chong ci ji THP-1xi bao chan sheng bao wai xian jing shi P38MAPK、ERK1/2MAPKxin hao tong lu lin suan hua shui ping shang sheng ,zhe zheng ming le yin dao mao di chong ci ji THP-1xi bao chan sheng de bao wai xian jing yu P38MAPK、ERK1/2MAPKxin hao tong lu you guan ;IL-1β、TNFαjian ce jie guo biao ming yin dao mao di chong ci ji THP-1xi bao chan sheng le IL-1β、TNFα,zhe zheng ming yin dao mao di chong ci ji THP-1xi bao chan sheng de bao wai xian jing ke neng yu IL-1β、TNFαyou guan ,ben yan jiu wei tan jiu yin dao mao di chong ci ji THP-1xi bao chan sheng bao wai xian jing de ji li di gong le xin sai lu 。
论文参考文献
论文详细介绍
论文作者分别是来自吉林大学的李飞，发表于刊物吉林大学2019-06-25论文，是一篇关于阴道毛滴虫论文,胞外陷阱论文,细胞论文,髓过氧化物酶论文,组蛋白论文,细胞因子论文,吉林大学2019-06-25论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自吉林大学2019-06-25论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：阴道毛滴虫论文; 胞外陷阱论文; 细胞论文; 髓过氧化物酶论文; 组蛋白论文; 细胞因子论文; 吉林大学2019-06-25论文;