本文主要研究内容
作者魏美林（2019）在《G6PDH调控的活性氧代谢在苯并噻重氮诱导苹果果实抗青霉病中的作用机理》一文中研究指出：本文以“金冠”苹果果实为材料,采后用苯并噻重氮（ASM）浸泡处理和不同浓度葡萄糖-6-磷酸脱氢酶（G6PDH）阻断剂脱氢异雄酮（DHEA）处理,研究常温贮藏期间损伤接种扩展青霉（Penicillium expansum）后果实病斑扩展、G6PDH活性及基因表达、活性氧（ROS）代谢以及品质指标的变化,主要结果如下:1.与对照相比,100 mg/L ASM处理显著抑制了苹果果实损伤接种P.expansum病斑的扩展,50μM DHEA结合ASM处理果实病斑扩展与单独ASM处理没有显著性差异,而100μM、200μM、400μM DHEA结合ASM处理均促进了果实病斑的扩展,其中以100μM DHEA处理对病斑直径扩展的促进作用最显著。2.ASM处理提高了苹果果肉和果皮中H2O2含量、NOX和SOD活性,并且上调了MdSOD基因表达。ASM+DHEA处理降低了贮藏前期果肉中H2O2积累,减少了果皮中H2O2含量,抑制果肉和果皮中NOX和SOD活性,并抑制MdSOD基因表达。3.ASM处理抑制了果肉中CAT活性,促进了果皮中CAT活性,提高了果肉和果皮中POD、GR、APX、MDHAR和DHAR活性,上调了MdAPX和MdDHAR基因表达,下调MdMDHAR基因表达,促进抗氧化物质AsA和GSH积累。ASM+DHEA处理消除了ASM对果肉中CAT活性的抑制,抑制了果皮中CAT活性,降低果肉和果皮中POD、GR、APX、MDHAR和DHAR活性及MdAPX基因表达,下调了果肉中MdDHAR基因表达,以及贮藏第8 d和第10 d果皮中MdDHAR基因表达。ASM+DHEA上调了果肉中MdMDHAR基因表达,但是进一步下调了果皮中MdMDHAR基因表达,使AsA和GSH含量维持在较低水平。4.ASM处理提高了果肉和果皮中G6PDH活性和NADPH含量,上调了果肉中MdG6PDH3、MdG6PDH4、MdG6PDH6基因表达以及果皮中MdG6PDH4在第4、6、10 d的表达,下调了果皮中MdG6PDH6表达。ASM+DHEA处理降低果肉和果皮中G6PDH活性和NADPH含量,上调果肉中MdG6PDH3和MdG6PDH6表达以及贮藏早期MdG6PDH4表达。与ASM处理相比,ASM+DHEA处理上调果皮中MdG6PDH6表达,下调第2、8、10 d果皮中MdG6PDH3表达。5.免疫荧光染色结果表明,贮藏第6 d,ASM处理显著促进了果肉细胞中G6PDH蛋白的积累,而ASM+DHEA处理降低了果肉细胞中G6PDH蛋白的积累。6.ASM处理对果实失重率的变化没有显著影响,但延缓了果肉硬度下降、抑制了呼吸强度和乙烯释放,降低可滴定酸（TA）积累,提高了果肉可溶性固形物（TSS）含量。ASM+DHEA处理延缓了果实失重率的增加,但是加快了果实硬度下降、提高TA含量、降低了TSS含量,促进呼吸强度和乙烯释放,并提前了呼吸高峰和乙烯高峰的出现。综上所述,ASM处理提高了MdG6PDH基因表达,增强了G6PDH活性和蛋白质积累,提高了NADPH含量,激活ROS代谢,提高了果实对青霉病的抗性,并保持较好的果实品质。而ASM+DHEA处理降低G6PDH蛋白积累和酶活性,抑制NADPH积累,抑制ROS代谢,降低果实对青霉病的抗性,并改变果实部分品质。由此说明,G6PDH调控的ROS代谢在ASM诱导苹果果实抗青霉病中具有重要作用。
Abstract
ben wen yi “jin guan ”ping guo guo shi wei cai liao ,cai hou yong ben bing sai chong dan （ASM）jin pao chu li he bu tong nong du pu tao tang -6-lin suan tuo qing mei （G6PDH）zu duan ji tuo qing yi xiong tong （DHEA）chu li ,yan jiu chang wen zhu cang ji jian sun shang jie chong kuo zhan qing mei （Penicillium expansum）hou guo shi bing ban kuo zhan 、G6PDHhuo xing ji ji yin biao da 、huo xing yang （ROS）dai xie yi ji pin zhi zhi biao de bian hua ,zhu yao jie guo ru xia :1.yu dui zhao xiang bi ,100 mg/L ASMchu li xian zhe yi zhi le ping guo guo shi sun shang jie chong P.expansumbing ban de kuo zhan ,50μM DHEAjie ge ASMchu li guo shi bing ban kuo zhan yu chan du ASMchu li mei you xian zhe xing cha yi ,er 100μM、200μM、400μM DHEAjie ge ASMchu li jun cu jin le guo shi bing ban de kuo zhan ,ji zhong yi 100μM DHEAchu li dui bing ban zhi jing kuo zhan de cu jin zuo yong zui xian zhe 。2.ASMchu li di gao le ping guo guo rou he guo pi zhong H2O2han liang 、NOXhe SODhuo xing ,bing ju shang diao le MdSODji yin biao da 。ASM+DHEAchu li jiang di le zhu cang qian ji guo rou zhong H2O2ji lei ,jian shao le guo pi zhong H2O2han liang ,yi zhi guo rou he guo pi zhong NOXhe SODhuo xing ,bing yi zhi MdSODji yin biao da 。3.ASMchu li yi zhi le guo rou zhong CAThuo xing ,cu jin le guo pi zhong CAThuo xing ,di gao le guo rou he guo pi zhong POD、GR、APX、MDHARhe DHARhuo xing ,shang diao le MdAPXhe MdDHARji yin biao da ,xia diao MdMDHARji yin biao da ,cu jin kang yang hua wu zhi AsAhe GSHji lei 。ASM+DHEAchu li xiao chu le ASMdui guo rou zhong CAThuo xing de yi zhi ,yi zhi le guo pi zhong CAThuo xing ,jiang di guo rou he guo pi zhong POD、GR、APX、MDHARhe DHARhuo xing ji MdAPXji yin biao da ,xia diao le guo rou zhong MdDHARji yin biao da ,yi ji zhu cang di 8 dhe di 10 dguo pi zhong MdDHARji yin biao da 。ASM+DHEAshang diao le guo rou zhong MdMDHARji yin biao da ,dan shi jin yi bu xia diao le guo pi zhong MdMDHARji yin biao da ,shi AsAhe GSHhan liang wei chi zai jiao di shui ping 。4.ASMchu li di gao le guo rou he guo pi zhong G6PDHhuo xing he NADPHhan liang ,shang diao le guo rou zhong MdG6PDH3、MdG6PDH4、MdG6PDH6ji yin biao da yi ji guo pi zhong MdG6PDH4zai di 4、6、10 dde biao da ,xia diao le guo pi zhong MdG6PDH6biao da 。ASM+DHEAchu li jiang di guo rou he guo pi zhong G6PDHhuo xing he NADPHhan liang ,shang diao guo rou zhong MdG6PDH3he MdG6PDH6biao da yi ji zhu cang zao ji MdG6PDH4biao da 。yu ASMchu li xiang bi ,ASM+DHEAchu li shang diao guo pi zhong MdG6PDH6biao da ,xia diao di 2、8、10 dguo pi zhong MdG6PDH3biao da 。5.mian yi ying guang ran se jie guo biao ming ,zhu cang di 6 d,ASMchu li xian zhe cu jin le guo rou xi bao zhong G6PDHdan bai de ji lei ,er ASM+DHEAchu li jiang di le guo rou xi bao zhong G6PDHdan bai de ji lei 。6.ASMchu li dui guo shi shi chong lv de bian hua mei you xian zhe ying xiang ,dan yan huan le guo rou ying du xia jiang 、yi zhi le hu xi jiang du he yi xi shi fang ,jiang di ke di ding suan （TA）ji lei ,di gao le guo rou ke rong xing gu xing wu （TSS）han liang 。ASM+DHEAchu li yan huan le guo shi shi chong lv de zeng jia ,dan shi jia kuai le guo shi ying du xia jiang 、di gao TAhan liang 、jiang di le TSShan liang ,cu jin hu xi jiang du he yi xi shi fang ,bing di qian le hu xi gao feng he yi xi gao feng de chu xian 。zeng shang suo shu ,ASMchu li di gao le MdG6PDHji yin biao da ,zeng jiang le G6PDHhuo xing he dan bai zhi ji lei ,di gao le NADPHhan liang ,ji huo ROSdai xie ,di gao le guo shi dui qing mei bing de kang xing ,bing bao chi jiao hao de guo shi pin zhi 。er ASM+DHEAchu li jiang di G6PDHdan bai ji lei he mei huo xing ,yi zhi NADPHji lei ,yi zhi ROSdai xie ,jiang di guo shi dui qing mei bing de kang xing ,bing gai bian guo shi bu fen pin zhi 。you ci shui ming ,G6PDHdiao kong de ROSdai xie zai ASMyou dao ping guo guo shi kang qing mei bing zhong ju you chong yao zuo yong 。
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论文作者分别是来自渤海大学的魏美林，发表于刊物渤海大学2019-10-16论文，是一篇关于苹果论文,苯并噻重氮论文,葡萄糖磷酸脱氢酶论文,诱导抗性论文,活性氧论文,渤海大学2019-10-16论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自渤海大学2019-10-16论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
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